![]() ![]() Mouse dragging: Click and hold down the left mouse button at the start position, and drag to the end position. Within the sequence viewer, there are several ways to select part of a sequence, or select a subset of sequences from a list, alignment or assembly: You can also pan in the Sequence View by holding Ctrl+Alt (command+Alt on Mac OS X) and clicking on the sequence and dragging. Hold the zoom key and click on an annotation to zoom to that annotation Hold the zoom key and turn the scroll wheel on your mouse (if you have one) to zoom in and out. Hold the zoom key and Shift key to zoom out. Hold the zoom key and left click on the sequence to zoom in. When the zoom key is pressed a magnifying glass mouse cursor will be displayed. Zooming can also be quickly achieved by holding down the zoom modifier key, which is the Ctrl key on Windows/Linux or the Alt/Option key on Mac OS X, and clicking as described below. ![]() ![]() Zooms out so as to fit the entire sequence in the available viewing area. The 100% zoom level allows for comfortable reading of the sequence. Zooms in to fit the selected region in the available viewing area. To zoom in or out by a smaller amount, hold down the alt and/or shift key while clicking the plus or minus button. The plus and minus buttons increase and decrease the magnification of the sequence by 50%, or by 30% if the magnification is already above 50%. Annotations, translations and analysis graphs are also displayed in this viewer.Ĭontrols for zooming in and out on sequences are located at the top of the side panel, to the right of the sequence viewer. Sequences are displayed in the viewer below the document table. To remove a sequence from a list entirely, select it and click the Delete button on your keyboard, or go to Delete under the Edit menu. This will copy each sequence out of the list into a separate document, while retaining the original sequence within the list. To extract sequences from a list, select the sequence(s) you want to extract and go to Sequence → Extract Sequences from List. Note that this copies your sequences into a list and retains the original sequence documents. To existing sequences in your database into a list, or combine two lists into one, select the sequences or sequence lists you want to group and go to Sequence → Group Sequences into a List. When you import files containing multiple sequences you will be asked if you want to store those sequences in a list. Sequence lists make it easier to manage large numbers of sequences by grouping related sequences into a single document. This will create a new sequence document containing the selected sequence. To create a new sequence from an existing sequence, select the region of sequence that you want then click the Extract button above the sequence viewer, or go to Sequence → Extract Regions. Bases not in the (green) binding region will be included as a 5′ extension. If your primer contains a 5′ extension, you can specify this by setting the length of the binding region. ![]() If your sequences are oligonucleotides, choose Primer or Probe as the type. You can change this by clicking the Type option. Geneious will automatically determine whether your sequence is nucleotide or protein based on the composition of the bases you enter. Here you can paste or type in the residues for your new sequence, then enter the Name, Description and Organism for your sequence if required. New sequences can be imported from existing files as described in Importing and Exporting Data or they can be created manually by going to Sequence → New Sequence, or File → New → Sequence. Creating, Viewing and Editing Sequences Creating new sequences ![]()
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